Precise Chromogenic Detection, Empowering Efficiency | EnkiLife IHC Detection Kits

Includes three models: mouse-specific (PA10014), rabbit-specific (PA10015), and mouse/rabbit universal (RA10006). This series uses mature and stable classical immunohistochemistry formulations, optimized through multiple batch process calibration, with a gentle and stable system and high fault tolerance, specifically adapted to conventional antigens and high/middle abundance protein staining.

The reagent components are scientifically proportioned, with strong performance compatibility among blocking solution, incubation solution, and chromogenic solution, effectively adapting to mainstream sample types such as FFPE paraffin sections and frozen sections. The overall operation workflow is standardized and easy to understand, without requiring complex debugging or special experimental conditions. Beginners can quickly get started, staining results are stable and uniform with minimal batch differences, and the ultra-high cost-effectiveness perfectly adapts to high-frequency basic scenarios such as daily batch laboratory experiments, teaching experiments, and routine sample screening, easily meeting basic experiment data retention and routine experiment needs.

Covers three models: mouse-specific (PA10016), rabbit-specific (PA10017), and mouse/rabbit universal (RA10007), which are high-precision research-exclusive reagents designed for low-abundance, difficult-to-stain, and easily missed target proteins. Different from traditional SP method and ABC method kits, this series adopts optimized and upgraded polymer HRP signal amplification technology, using high-molecular-weight polymerase-labeled structure, greatly improving enzyme binding efficiency and signal amplification multiples, with signal gain effect far exceeding traditional reagents.

It can precisely capture target proteins with trace expression, transient expression, and inducible expression in tissues, solving common problems of traditional reagents such as weak signal loss, false negatives, dim staining, and blurred edges. Meanwhile, the reagent has a built-in efficient blocking system that can effectively block endogenous peroxidase, biotin, and non-specific protein interference in tissues, greatly reducing background staining with extremely strong specificity, especially suitable for high-precision staining research of difficult-to-detect targets such as tumor microenvironment markers, cytokines, and signaling pathway proteins.

Includes universal double staining kit (PA10001) and Polymer-HRP high-sensitivity double staining kit (PA10002), using the classic DAB/AEC dual-color chromogenic system recognized by the research community, with DAB showing brownish-yellow and AEC showing red. The dual-color difference is clear and distinctive with no cross-color interference. The reagent system has been specially optimized, compatible with both one-step synchronous staining and stepwise staining modes, achieving simultaneous localization and expression analysis of two target proteins on the same tissue section.

Compared to traditional single staining experiments that require comparison of multiple sections, the double staining kit greatly saves precious tissue samples, reduces repeated operations such as sectioning, incubation, and washing, shortens the experiment cycle by nearly half, while intuitively comparing the expression location, expression level difference, and co-expression relationship of two proteins, greatly assisting multi-target combined research such as tumor classification, cell co-localization, target interaction, and pathological control analysis, making experimental data more comparative and persuasive.

Specifically launched in two acid-base adapted models: pH 6.0 (PA10020) and pH 9.0 (PA10021). The core innovation of the product is the one-step deparaffinization/antigen retrieval system, breaking the cumbersome workflow of traditional paraffin sections that require stepwise deparaffinization, gradient hydration, and high-temperature antigen retrieval, efficiently integrating multiple steps into one. No multiple solution changes needed, no high-temperature assistance needed, greatly streamlining operation steps, effectively shortening the overall experiment duration, and significantly improving experiment efficiency. Meanwhile, it greatly reduces manual operation intervention, lowers human errors, effectively improves staining consistency and reproducibility across different batches and operation scenarios, making paraffin IHC experiment results more stable and standardized.

Safety and eco-friendliness is one of the core highlights of this product. Its dedicated retrieval solution uses a xylene-free eco-friendly formulation, without highly toxic volatile organic solvents, abandoning the toxic and harmful drawbacks of traditional reagents. The experiment can be operated without relying on fume hoods, effectively protecting experimenter health, optimizing the laboratory operation environment, while lowering experiment pollution and eco-treatment costs, achieving a dual balance of efficient experiments and green safety. The kit is equipped with a complete set of supporting reagents, including endogenous peroxidase blocker, dedicated antibody diluent, high-sensitivity Polymer-HRP secondary antibody, DAB chromogenic solution, and hematoxylin counterstaining solution, requiring no additional supporting reagents, truly achieving a one-stop complete solution.